Western Blot:Article Title: Geniposide alleviates kidney fibrosis by targeting STAT3-HK2-mediated glycolysis.
Article Snippet: Its molecular formula is C17 H24O10, and its structural formula is depicted in Fig. 1A.Its molecular formula is C17 H24O10, and its structural formula is depicted in Fig. 1A.. Primary antibodies against smooth muscle actin (α-SMA) (Cat: 14395-1-AP), vimentin (Cat: 60330-1-Ig), E-cadherin (Cat: 20874-1-AP), STAT3 (Cat: 10253-2-AP), HK2 (Cat: 66974-1-Ig), PPARα (Cat: 66826-1-Ig) and α-tubulin (Cat: 11224-1-AP) and HRPconjugated secondary antibodies against mice (Cat: RGAM001) and rabbits (Cat: RGAR001) were obtained from Proteintech Group (Chicago, IL, USA).. The anticollagen I primary antibody (Cat: ab260043), anti-pSTAT3 (Cat: ab76315), anti-ACOX1 (Cat: ab184032), anti-CPT1 (Cat: ab234111) and recombinant human transforming growth factor β1 (TGF-β) (Cat: ab50036) were obtained from Abcam (Cambridge, UK).The anticollagen I primary antibody (Cat: ab260043), anti-pSTAT3 (Cat: ab76315), anti-ACOX1 (Cat: ab184032), anti-CPT1 (Cat: ab234111) and recombinant human transforming growth factor β1 (TGF-β) (Cat: ab50036) were obtained from Abcam (Cambridge, UK).
Article Title: Geniposide alleviates kidney fibrosis by targeting STAT3-HK2-mediated glycolysis
Article Snippet: Its molecular formula is C 17 H24O10, and its structural formula is depicted in Fig. A.Its molecular formula is C 17 H24O10, and its structural formula is depicted in Fig. A.. Primary antibodies against smooth muscle actin (α-SMA) (Cat: 14395-1-AP), vimentin (Cat: 60330-1-Ig), E-cadherin (Cat: 20874-1-AP), STAT3 (Cat: 10253-2-AP), HK2 (Cat: 66974-1-Ig), PPARα (Cat: 66826-1-Ig) and α-tubulin (Cat: 11224-1-AP) and HRP-conjugated secondary antibodies against mice (Cat: RGAM001) and rabbits (Cat: RGAR001) were obtained from Proteintech Group (Chicago, IL, USA).. The anti-collagen I primary antibody (Cat: ab260043), anti-p-STAT3 (Cat: ab76315), anti-ACOX1 (Cat: ab184032), anti-CPT1 (Cat: ab234111) and recombinant human transforming growth factor β1 (TGF-β) (Cat: ab50036) were obtained from Abcam (Cambridge, UK).The anti-collagen I primary antibody (Cat: ab260043), anti-p-STAT3 (Cat: ab76315), anti-ACOX1 (Cat: ab184032), anti-CPT1 (Cat: ab234111) and recombinant human transforming growth factor β1 (TGF-β) (Cat: ab50036) were obtained from Abcam (Cambridge, UK).
Immunohistochemical staining:Article Title: Geniposide alleviates kidney fibrosis by targeting STAT3-HK2-mediated glycolysis.
Article Snippet: Its molecular formula is C17 H24O10, and its structural formula is depicted in Fig. 1A.Its molecular formula is C17 H24O10, and its structural formula is depicted in Fig. 1A.. Primary antibodies against smooth muscle actin (α-SMA) (Cat: 14395-1-AP), vimentin (Cat: 60330-1-Ig), E-cadherin (Cat: 20874-1-AP), STAT3 (Cat: 10253-2-AP), HK2 (Cat: 66974-1-Ig), PPARα (Cat: 66826-1-Ig) and α-tubulin (Cat: 11224-1-AP) and HRPconjugated secondary antibodies against mice (Cat: RGAM001) and rabbits (Cat: RGAR001) were obtained from Proteintech Group (Chicago, IL, USA).. The anticollagen I primary antibody (Cat: ab260043), anti-pSTAT3 (Cat: ab76315), anti-ACOX1 (Cat: ab184032), anti-CPT1 (Cat: ab234111) and recombinant human transforming growth factor β1 (TGF-β) (Cat: ab50036) were obtained from Abcam (Cambridge, UK).The anticollagen I primary antibody (Cat: ab260043), anti-pSTAT3 (Cat: ab76315), anti-ACOX1 (Cat: ab184032), anti-CPT1 (Cat: ab234111) and recombinant human transforming growth factor β1 (TGF-β) (Cat: ab50036) were obtained from Abcam (Cambridge, UK).
Article Title: Geniposide alleviates kidney fibrosis by targeting STAT3-HK2-mediated glycolysis
Article Snippet: Its molecular formula is C 17 H24O10, and its structural formula is depicted in Fig. A.Its molecular formula is C 17 H24O10, and its structural formula is depicted in Fig. A.. Primary antibodies against smooth muscle actin (α-SMA) (Cat: 14395-1-AP), vimentin (Cat: 60330-1-Ig), E-cadherin (Cat: 20874-1-AP), STAT3 (Cat: 10253-2-AP), HK2 (Cat: 66974-1-Ig), PPARα (Cat: 66826-1-Ig) and α-tubulin (Cat: 11224-1-AP) and HRP-conjugated secondary antibodies against mice (Cat: RGAM001) and rabbits (Cat: RGAR001) were obtained from Proteintech Group (Chicago, IL, USA).. The anti-collagen I primary antibody (Cat: ab260043), anti-p-STAT3 (Cat: ab76315), anti-ACOX1 (Cat: ab184032), anti-CPT1 (Cat: ab234111) and recombinant human transforming growth factor β1 (TGF-β) (Cat: ab50036) were obtained from Abcam (Cambridge, UK).The anti-collagen I primary antibody (Cat: ab260043), anti-p-STAT3 (Cat: ab76315), anti-ACOX1 (Cat: ab184032), anti-CPT1 (Cat: ab234111) and recombinant human transforming growth factor β1 (TGF-β) (Cat: ab50036) were obtained from Abcam (Cambridge, UK).
Staining:Article Title: Geniposide alleviates kidney fibrosis by targeting STAT3-HK2-mediated glycolysis.
Article Snippet: Its molecular formula is C17 H24O10, and its structural formula is depicted in Fig. 1A.Its molecular formula is C17 H24O10, and its structural formula is depicted in Fig. 1A.. Primary antibodies against smooth muscle actin (α-SMA) (Cat: 14395-1-AP), vimentin (Cat: 60330-1-Ig), E-cadherin (Cat: 20874-1-AP), STAT3 (Cat: 10253-2-AP), HK2 (Cat: 66974-1-Ig), PPARα (Cat: 66826-1-Ig) and α-tubulin (Cat: 11224-1-AP) and HRPconjugated secondary antibodies against mice (Cat: RGAM001) and rabbits (Cat: RGAR001) were obtained from Proteintech Group (Chicago, IL, USA).. The anticollagen I primary antibody (Cat: ab260043), anti-pSTAT3 (Cat: ab76315), anti-ACOX1 (Cat: ab184032), anti-CPT1 (Cat: ab234111) and recombinant human transforming growth factor β1 (TGF-β) (Cat: ab50036) were obtained from Abcam (Cambridge, UK).The anticollagen I primary antibody (Cat: ab260043), anti-pSTAT3 (Cat: ab76315), anti-ACOX1 (Cat: ab184032), anti-CPT1 (Cat: ab234111) and recombinant human transforming growth factor β1 (TGF-β) (Cat: ab50036) were obtained from Abcam (Cambridge, UK).
Article Title: Geniposide alleviates kidney fibrosis by targeting STAT3-HK2-mediated glycolysis
Article Snippet: Its molecular formula is C 17 H24O10, and its structural formula is depicted in Fig. A.Its molecular formula is C 17 H24O10, and its structural formula is depicted in Fig. A.. Primary antibodies against smooth muscle actin (α-SMA) (Cat: 14395-1-AP), vimentin (Cat: 60330-1-Ig), E-cadherin (Cat: 20874-1-AP), STAT3 (Cat: 10253-2-AP), HK2 (Cat: 66974-1-Ig), PPARα (Cat: 66826-1-Ig) and α-tubulin (Cat: 11224-1-AP) and HRP-conjugated secondary antibodies against mice (Cat: RGAM001) and rabbits (Cat: RGAR001) were obtained from Proteintech Group (Chicago, IL, USA).. The anti-collagen I primary antibody (Cat: ab260043), anti-p-STAT3 (Cat: ab76315), anti-ACOX1 (Cat: ab184032), anti-CPT1 (Cat: ab234111) and recombinant human transforming growth factor β1 (TGF-β) (Cat: ab50036) were obtained from Abcam (Cambridge, UK).The anti-collagen I primary antibody (Cat: ab260043), anti-p-STAT3 (Cat: ab76315), anti-ACOX1 (Cat: ab184032), anti-CPT1 (Cat: ab234111) and recombinant human transforming growth factor β1 (TGF-β) (Cat: ab50036) were obtained from Abcam (Cambridge, UK).
Marker:Article Title: Geniposide alleviates kidney fibrosis by targeting STAT3-HK2-mediated glycolysis.
Article Snippet: Its molecular formula is C17 H24O10, and its structural formula is depicted in Fig. 1A.Its molecular formula is C17 H24O10, and its structural formula is depicted in Fig. 1A.. Primary antibodies against smooth muscle actin (α-SMA) (Cat: 14395-1-AP), vimentin (Cat: 60330-1-Ig), E-cadherin (Cat: 20874-1-AP), STAT3 (Cat: 10253-2-AP), HK2 (Cat: 66974-1-Ig), PPARα (Cat: 66826-1-Ig) and α-tubulin (Cat: 11224-1-AP) and HRPconjugated secondary antibodies against mice (Cat: RGAM001) and rabbits (Cat: RGAR001) were obtained from Proteintech Group (Chicago, IL, USA).. The anticollagen I primary antibody (Cat: ab260043), anti-pSTAT3 (Cat: ab76315), anti-ACOX1 (Cat: ab184032), anti-CPT1 (Cat: ab234111) and recombinant human transforming growth factor β1 (TGF-β) (Cat: ab50036) were obtained from Abcam (Cambridge, UK).The anticollagen I primary antibody (Cat: ab260043), anti-pSTAT3 (Cat: ab76315), anti-ACOX1 (Cat: ab184032), anti-CPT1 (Cat: ab234111) and recombinant human transforming growth factor β1 (TGF-β) (Cat: ab50036) were obtained from Abcam (Cambridge, UK).
Article Title: Geniposide alleviates kidney fibrosis by targeting STAT3-HK2-mediated glycolysis
Article Snippet: Its molecular formula is C 17 H24O10, and its structural formula is depicted in Fig. A.Its molecular formula is C 17 H24O10, and its structural formula is depicted in Fig. A.. Primary antibodies against smooth muscle actin (α-SMA) (Cat: 14395-1-AP), vimentin (Cat: 60330-1-Ig), E-cadherin (Cat: 20874-1-AP), STAT3 (Cat: 10253-2-AP), HK2 (Cat: 66974-1-Ig), PPARα (Cat: 66826-1-Ig) and α-tubulin (Cat: 11224-1-AP) and HRP-conjugated secondary antibodies against mice (Cat: RGAM001) and rabbits (Cat: RGAR001) were obtained from Proteintech Group (Chicago, IL, USA).. The anti-collagen I primary antibody (Cat: ab260043), anti-p-STAT3 (Cat: ab76315), anti-ACOX1 (Cat: ab184032), anti-CPT1 (Cat: ab234111) and recombinant human transforming growth factor β1 (TGF-β) (Cat: ab50036) were obtained from Abcam (Cambridge, UK).The anti-collagen I primary antibody (Cat: ab260043), anti-p-STAT3 (Cat: ab76315), anti-ACOX1 (Cat: ab184032), anti-CPT1 (Cat: ab234111) and recombinant human transforming growth factor β1 (TGF-β) (Cat: ab50036) were obtained from Abcam (Cambridge, UK).
Wound Healing Assay:Article Title: Geniposide alleviates kidney fibrosis by targeting STAT3-HK2-mediated glycolysis.
Article Snippet: Its molecular formula is C17 H24O10, and its structural formula is depicted in Fig. 1A.Its molecular formula is C17 H24O10, and its structural formula is depicted in Fig. 1A.. Primary antibodies against smooth muscle actin (α-SMA) (Cat: 14395-1-AP), vimentin (Cat: 60330-1-Ig), E-cadherin (Cat: 20874-1-AP), STAT3 (Cat: 10253-2-AP), HK2 (Cat: 66974-1-Ig), PPARα (Cat: 66826-1-Ig) and α-tubulin (Cat: 11224-1-AP) and HRPconjugated secondary antibodies against mice (Cat: RGAM001) and rabbits (Cat: RGAR001) were obtained from Proteintech Group (Chicago, IL, USA).. The anticollagen I primary antibody (Cat: ab260043), anti-pSTAT3 (Cat: ab76315), anti-ACOX1 (Cat: ab184032), anti-CPT1 (Cat: ab234111) and recombinant human transforming growth factor β1 (TGF-β) (Cat: ab50036) were obtained from Abcam (Cambridge, UK).The anticollagen I primary antibody (Cat: ab260043), anti-pSTAT3 (Cat: ab76315), anti-ACOX1 (Cat: ab184032), anti-CPT1 (Cat: ab234111) and recombinant human transforming growth factor β1 (TGF-β) (Cat: ab50036) were obtained from Abcam (Cambridge, UK).
Article Title: Geniposide alleviates kidney fibrosis by targeting STAT3-HK2-mediated glycolysis
Article Snippet: Its molecular formula is C 17 H24O10, and its structural formula is depicted in Fig. A.Its molecular formula is C 17 H24O10, and its structural formula is depicted in Fig. A.. Primary antibodies against smooth muscle actin (α-SMA) (Cat: 14395-1-AP), vimentin (Cat: 60330-1-Ig), E-cadherin (Cat: 20874-1-AP), STAT3 (Cat: 10253-2-AP), HK2 (Cat: 66974-1-Ig), PPARα (Cat: 66826-1-Ig) and α-tubulin (Cat: 11224-1-AP) and HRP-conjugated secondary antibodies against mice (Cat: RGAM001) and rabbits (Cat: RGAR001) were obtained from Proteintech Group (Chicago, IL, USA).. The anti-collagen I primary antibody (Cat: ab260043), anti-p-STAT3 (Cat: ab76315), anti-ACOX1 (Cat: ab184032), anti-CPT1 (Cat: ab234111) and recombinant human transforming growth factor β1 (TGF-β) (Cat: ab50036) were obtained from Abcam (Cambridge, UK).The anti-collagen I primary antibody (Cat: ab260043), anti-p-STAT3 (Cat: ab76315), anti-ACOX1 (Cat: ab184032), anti-CPT1 (Cat: ab234111) and recombinant human transforming growth factor β1 (TGF-β) (Cat: ab50036) were obtained from Abcam (Cambridge, UK).
Migration:Article Title: Geniposide alleviates kidney fibrosis by targeting STAT3-HK2-mediated glycolysis.
Article Snippet: Its molecular formula is C17 H24O10, and its structural formula is depicted in Fig. 1A.Its molecular formula is C17 H24O10, and its structural formula is depicted in Fig. 1A.. Primary antibodies against smooth muscle actin (α-SMA) (Cat: 14395-1-AP), vimentin (Cat: 60330-1-Ig), E-cadherin (Cat: 20874-1-AP), STAT3 (Cat: 10253-2-AP), HK2 (Cat: 66974-1-Ig), PPARα (Cat: 66826-1-Ig) and α-tubulin (Cat: 11224-1-AP) and HRPconjugated secondary antibodies against mice (Cat: RGAM001) and rabbits (Cat: RGAR001) were obtained from Proteintech Group (Chicago, IL, USA).. The anticollagen I primary antibody (Cat: ab260043), anti-pSTAT3 (Cat: ab76315), anti-ACOX1 (Cat: ab184032), anti-CPT1 (Cat: ab234111) and recombinant human transforming growth factor β1 (TGF-β) (Cat: ab50036) were obtained from Abcam (Cambridge, UK).The anticollagen I primary antibody (Cat: ab260043), anti-pSTAT3 (Cat: ab76315), anti-ACOX1 (Cat: ab184032), anti-CPT1 (Cat: ab234111) and recombinant human transforming growth factor β1 (TGF-β) (Cat: ab50036) were obtained from Abcam (Cambridge, UK).
Article Title: Geniposide alleviates kidney fibrosis by targeting STAT3-HK2-mediated glycolysis
Article Snippet: Its molecular formula is C 17 H24O10, and its structural formula is depicted in Fig. A.Its molecular formula is C 17 H24O10, and its structural formula is depicted in Fig. A.. Primary antibodies against smooth muscle actin (α-SMA) (Cat: 14395-1-AP), vimentin (Cat: 60330-1-Ig), E-cadherin (Cat: 20874-1-AP), STAT3 (Cat: 10253-2-AP), HK2 (Cat: 66974-1-Ig), PPARα (Cat: 66826-1-Ig) and α-tubulin (Cat: 11224-1-AP) and HRP-conjugated secondary antibodies against mice (Cat: RGAM001) and rabbits (Cat: RGAR001) were obtained from Proteintech Group (Chicago, IL, USA).. The anti-collagen I primary antibody (Cat: ab260043), anti-p-STAT3 (Cat: ab76315), anti-ACOX1 (Cat: ab184032), anti-CPT1 (Cat: ab234111) and recombinant human transforming growth factor β1 (TGF-β) (Cat: ab50036) were obtained from Abcam (Cambridge, UK).The anti-collagen I primary antibody (Cat: ab260043), anti-p-STAT3 (Cat: ab76315), anti-ACOX1 (Cat: ab184032), anti-CPT1 (Cat: ab234111) and recombinant human transforming growth factor β1 (TGF-β) (Cat: ab50036) were obtained from Abcam (Cambridge, UK).
Expressing:Article Title: Geniposide alleviates kidney fibrosis by targeting STAT3-HK2-mediated glycolysis.
Article Snippet: Its molecular formula is C17 H24O10, and its structural formula is depicted in Fig. 1A.Its molecular formula is C17 H24O10, and its structural formula is depicted in Fig. 1A.. Primary antibodies against smooth muscle actin (α-SMA) (Cat: 14395-1-AP), vimentin (Cat: 60330-1-Ig), E-cadherin (Cat: 20874-1-AP), STAT3 (Cat: 10253-2-AP), HK2 (Cat: 66974-1-Ig), PPARα (Cat: 66826-1-Ig) and α-tubulin (Cat: 11224-1-AP) and HRPconjugated secondary antibodies against mice (Cat: RGAM001) and rabbits (Cat: RGAR001) were obtained from Proteintech Group (Chicago, IL, USA).. The anticollagen I primary antibody (Cat: ab260043), anti-pSTAT3 (Cat: ab76315), anti-ACOX1 (Cat: ab184032), anti-CPT1 (Cat: ab234111) and recombinant human transforming growth factor β1 (TGF-β) (Cat: ab50036) were obtained from Abcam (Cambridge, UK).The anticollagen I primary antibody (Cat: ab260043), anti-pSTAT3 (Cat: ab76315), anti-ACOX1 (Cat: ab184032), anti-CPT1 (Cat: ab234111) and recombinant human transforming growth factor β1 (TGF-β) (Cat: ab50036) were obtained from Abcam (Cambridge, UK).
Article Title: Geniposide alleviates kidney fibrosis by targeting STAT3-HK2-mediated glycolysis
Article Snippet: Its molecular formula is C 17 H24O10, and its structural formula is depicted in Fig. A.Its molecular formula is C 17 H24O10, and its structural formula is depicted in Fig. A.. Primary antibodies against smooth muscle actin (α-SMA) (Cat: 14395-1-AP), vimentin (Cat: 60330-1-Ig), E-cadherin (Cat: 20874-1-AP), STAT3 (Cat: 10253-2-AP), HK2 (Cat: 66974-1-Ig), PPARα (Cat: 66826-1-Ig) and α-tubulin (Cat: 11224-1-AP) and HRP-conjugated secondary antibodies against mice (Cat: RGAM001) and rabbits (Cat: RGAR001) were obtained from Proteintech Group (Chicago, IL, USA).. The anti-collagen I primary antibody (Cat: ab260043), anti-p-STAT3 (Cat: ab76315), anti-ACOX1 (Cat: ab184032), anti-CPT1 (Cat: ab234111) and recombinant human transforming growth factor β1 (TGF-β) (Cat: ab50036) were obtained from Abcam (Cambridge, UK).The anti-collagen I primary antibody (Cat: ab260043), anti-p-STAT3 (Cat: ab76315), anti-ACOX1 (Cat: ab184032), anti-CPT1 (Cat: ab234111) and recombinant human transforming growth factor β1 (TGF-β) (Cat: ab50036) were obtained from Abcam (Cambridge, UK).
Immunofluorescence:Article Title: Geniposide alleviates kidney fibrosis by targeting STAT3-HK2-mediated glycolysis.
Article Snippet: Its molecular formula is C17 H24O10, and its structural formula is depicted in Fig. 1A.Its molecular formula is C17 H24O10, and its structural formula is depicted in Fig. 1A.. Primary antibodies against smooth muscle actin (α-SMA) (Cat: 14395-1-AP), vimentin (Cat: 60330-1-Ig), E-cadherin (Cat: 20874-1-AP), STAT3 (Cat: 10253-2-AP), HK2 (Cat: 66974-1-Ig), PPARα (Cat: 66826-1-Ig) and α-tubulin (Cat: 11224-1-AP) and HRPconjugated secondary antibodies against mice (Cat: RGAM001) and rabbits (Cat: RGAR001) were obtained from Proteintech Group (Chicago, IL, USA).. The anticollagen I primary antibody (Cat: ab260043), anti-pSTAT3 (Cat: ab76315), anti-ACOX1 (Cat: ab184032), anti-CPT1 (Cat: ab234111) and recombinant human transforming growth factor β1 (TGF-β) (Cat: ab50036) were obtained from Abcam (Cambridge, UK).The anticollagen I primary antibody (Cat: ab260043), anti-pSTAT3 (Cat: ab76315), anti-ACOX1 (Cat: ab184032), anti-CPT1 (Cat: ab234111) and recombinant human transforming growth factor β1 (TGF-β) (Cat: ab50036) were obtained from Abcam (Cambridge, UK).
Article Title: Geniposide alleviates kidney fibrosis by targeting STAT3-HK2-mediated glycolysis
Article Snippet: Its molecular formula is C 17 H24O10, and its structural formula is depicted in Fig. A.Its molecular formula is C 17 H24O10, and its structural formula is depicted in Fig. A.. Primary antibodies against smooth muscle actin (α-SMA) (Cat: 14395-1-AP), vimentin (Cat: 60330-1-Ig), E-cadherin (Cat: 20874-1-AP), STAT3 (Cat: 10253-2-AP), HK2 (Cat: 66974-1-Ig), PPARα (Cat: 66826-1-Ig) and α-tubulin (Cat: 11224-1-AP) and HRP-conjugated secondary antibodies against mice (Cat: RGAM001) and rabbits (Cat: RGAR001) were obtained from Proteintech Group (Chicago, IL, USA).. The anti-collagen I primary antibody (Cat: ab260043), anti-p-STAT3 (Cat: ab76315), anti-ACOX1 (Cat: ab184032), anti-CPT1 (Cat: ab234111) and recombinant human transforming growth factor β1 (TGF-β) (Cat: ab50036) were obtained from Abcam (Cambridge, UK).The anti-collagen I primary antibody (Cat: ab260043), anti-p-STAT3 (Cat: ab76315), anti-ACOX1 (Cat: ab184032), anti-CPT1 (Cat: ab234111) and recombinant human transforming growth factor β1 (TGF-β) (Cat: ab50036) were obtained from Abcam (Cambridge, UK).
Transfection:Article Title: Geniposide alleviates kidney fibrosis by targeting STAT3-HK2-mediated glycolysis.
Article Snippet: Its molecular formula is C17 H24O10, and its structural formula is depicted in Fig. 1A.Its molecular formula is C17 H24O10, and its structural formula is depicted in Fig. 1A.. Primary antibodies against smooth muscle actin (α-SMA) (Cat: 14395-1-AP), vimentin (Cat: 60330-1-Ig), E-cadherin (Cat: 20874-1-AP), STAT3 (Cat: 10253-2-AP), HK2 (Cat: 66974-1-Ig), PPARα (Cat: 66826-1-Ig) and α-tubulin (Cat: 11224-1-AP) and HRPconjugated secondary antibodies against mice (Cat: RGAM001) and rabbits (Cat: RGAR001) were obtained from Proteintech Group (Chicago, IL, USA).. The anticollagen I primary antibody (Cat: ab260043), anti-pSTAT3 (Cat: ab76315), anti-ACOX1 (Cat: ab184032), anti-CPT1 (Cat: ab234111) and recombinant human transforming growth factor β1 (TGF-β) (Cat: ab50036) were obtained from Abcam (Cambridge, UK).The anticollagen I primary antibody (Cat: ab260043), anti-pSTAT3 (Cat: ab76315), anti-ACOX1 (Cat: ab184032), anti-CPT1 (Cat: ab234111) and recombinant human transforming growth factor β1 (TGF-β) (Cat: ab50036) were obtained from Abcam (Cambridge, UK).
Article Title: Geniposide alleviates kidney fibrosis by targeting STAT3-HK2-mediated glycolysis
Article Snippet: Its molecular formula is C 17 H24O10, and its structural formula is depicted in Fig. A.Its molecular formula is C 17 H24O10, and its structural formula is depicted in Fig. A.. Primary antibodies against smooth muscle actin (α-SMA) (Cat: 14395-1-AP), vimentin (Cat: 60330-1-Ig), E-cadherin (Cat: 20874-1-AP), STAT3 (Cat: 10253-2-AP), HK2 (Cat: 66974-1-Ig), PPARα (Cat: 66826-1-Ig) and α-tubulin (Cat: 11224-1-AP) and HRP-conjugated secondary antibodies against mice (Cat: RGAM001) and rabbits (Cat: RGAR001) were obtained from Proteintech Group (Chicago, IL, USA).. The anti-collagen I primary antibody (Cat: ab260043), anti-p-STAT3 (Cat: ab76315), anti-ACOX1 (Cat: ab184032), anti-CPT1 (Cat: ab234111) and recombinant human transforming growth factor β1 (TGF-β) (Cat: ab50036) were obtained from Abcam (Cambridge, UK).The anti-collagen I primary antibody (Cat: ab260043), anti-p-STAT3 (Cat: ab76315), anti-ACOX1 (Cat: ab184032), anti-CPT1 (Cat: ab234111) and recombinant human transforming growth factor β1 (TGF-β) (Cat: ab50036) were obtained from Abcam (Cambridge, UK).
Plasmid Preparation:Article Title: Geniposide alleviates kidney fibrosis by targeting STAT3-HK2-mediated glycolysis.
Article Snippet: Its molecular formula is C17 H24O10, and its structural formula is depicted in Fig. 1A.Its molecular formula is C17 H24O10, and its structural formula is depicted in Fig. 1A.. Primary antibodies against smooth muscle actin (α-SMA) (Cat: 14395-1-AP), vimentin (Cat: 60330-1-Ig), E-cadherin (Cat: 20874-1-AP), STAT3 (Cat: 10253-2-AP), HK2 (Cat: 66974-1-Ig), PPARα (Cat: 66826-1-Ig) and α-tubulin (Cat: 11224-1-AP) and HRPconjugated secondary antibodies against mice (Cat: RGAM001) and rabbits (Cat: RGAR001) were obtained from Proteintech Group (Chicago, IL, USA).. The anticollagen I primary antibody (Cat: ab260043), anti-pSTAT3 (Cat: ab76315), anti-ACOX1 (Cat: ab184032), anti-CPT1 (Cat: ab234111) and recombinant human transforming growth factor β1 (TGF-β) (Cat: ab50036) were obtained from Abcam (Cambridge, UK).The anticollagen I primary antibody (Cat: ab260043), anti-pSTAT3 (Cat: ab76315), anti-ACOX1 (Cat: ab184032), anti-CPT1 (Cat: ab234111) and recombinant human transforming growth factor β1 (TGF-β) (Cat: ab50036) were obtained from Abcam (Cambridge, UK).
Article Title: Geniposide alleviates kidney fibrosis by targeting STAT3-HK2-mediated glycolysis
Article Snippet: Its molecular formula is C 17 H24O10, and its structural formula is depicted in Fig. A.Its molecular formula is C 17 H24O10, and its structural formula is depicted in Fig. A.. Primary antibodies against smooth muscle actin (α-SMA) (Cat: 14395-1-AP), vimentin (Cat: 60330-1-Ig), E-cadherin (Cat: 20874-1-AP), STAT3 (Cat: 10253-2-AP), HK2 (Cat: 66974-1-Ig), PPARα (Cat: 66826-1-Ig) and α-tubulin (Cat: 11224-1-AP) and HRP-conjugated secondary antibodies against mice (Cat: RGAM001) and rabbits (Cat: RGAR001) were obtained from Proteintech Group (Chicago, IL, USA).. The anti-collagen I primary antibody (Cat: ab260043), anti-p-STAT3 (Cat: ab76315), anti-ACOX1 (Cat: ab184032), anti-CPT1 (Cat: ab234111) and recombinant human transforming growth factor β1 (TGF-β) (Cat: ab50036) were obtained from Abcam (Cambridge, UK).The anti-collagen I primary antibody (Cat: ab260043), anti-p-STAT3 (Cat: ab76315), anti-ACOX1 (Cat: ab184032), anti-CPT1 (Cat: ab234111) and recombinant human transforming growth factor β1 (TGF-β) (Cat: ab50036) were obtained from Abcam (Cambridge, UK).
Control:Article Title: Geniposide alleviates kidney fibrosis by targeting STAT3-HK2-mediated glycolysis.
Article Snippet: Its molecular formula is C17 H24O10, and its structural formula is depicted in Fig. 1A.Its molecular formula is C17 H24O10, and its structural formula is depicted in Fig. 1A.. Primary antibodies against smooth muscle actin (α-SMA) (Cat: 14395-1-AP), vimentin (Cat: 60330-1-Ig), E-cadherin (Cat: 20874-1-AP), STAT3 (Cat: 10253-2-AP), HK2 (Cat: 66974-1-Ig), PPARα (Cat: 66826-1-Ig) and α-tubulin (Cat: 11224-1-AP) and HRPconjugated secondary antibodies against mice (Cat: RGAM001) and rabbits (Cat: RGAR001) were obtained from Proteintech Group (Chicago, IL, USA).. The anticollagen I primary antibody (Cat: ab260043), anti-pSTAT3 (Cat: ab76315), anti-ACOX1 (Cat: ab184032), anti-CPT1 (Cat: ab234111) and recombinant human transforming growth factor β1 (TGF-β) (Cat: ab50036) were obtained from Abcam (Cambridge, UK).The anticollagen I primary antibody (Cat: ab260043), anti-pSTAT3 (Cat: ab76315), anti-ACOX1 (Cat: ab184032), anti-CPT1 (Cat: ab234111) and recombinant human transforming growth factor β1 (TGF-β) (Cat: ab50036) were obtained from Abcam (Cambridge, UK).
Article Title: Geniposide alleviates kidney fibrosis by targeting STAT3-HK2-mediated glycolysis
Article Snippet: Its molecular formula is C 17 H24O10, and its structural formula is depicted in Fig. A.Its molecular formula is C 17 H24O10, and its structural formula is depicted in Fig. A.. Primary antibodies against smooth muscle actin (α-SMA) (Cat: 14395-1-AP), vimentin (Cat: 60330-1-Ig), E-cadherin (Cat: 20874-1-AP), STAT3 (Cat: 10253-2-AP), HK2 (Cat: 66974-1-Ig), PPARα (Cat: 66826-1-Ig) and α-tubulin (Cat: 11224-1-AP) and HRP-conjugated secondary antibodies against mice (Cat: RGAM001) and rabbits (Cat: RGAR001) were obtained from Proteintech Group (Chicago, IL, USA).. The anti-collagen I primary antibody (Cat: ab260043), anti-p-STAT3 (Cat: ab76315), anti-ACOX1 (Cat: ab184032), anti-CPT1 (Cat: ab234111) and recombinant human transforming growth factor β1 (TGF-β) (Cat: ab50036) were obtained from Abcam (Cambridge, UK).The anti-collagen I primary antibody (Cat: ab260043), anti-p-STAT3 (Cat: ab76315), anti-ACOX1 (Cat: ab184032), anti-CPT1 (Cat: ab234111) and recombinant human transforming growth factor β1 (TGF-β) (Cat: ab50036) were obtained from Abcam (Cambridge, UK).
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